Bacteriological analysis of drinking water pages with reference to book, from 92 to 96 zumra sami public health division, national institute of health, islamabad. Full text full text is available as a scanned copy of the original print version. This note presents the background to the testing of water samples to determine whether diseasecausing bacteria, in. In this study a total of 22 drinking water samples, from the.
The procedure followed is fundamental to bacteriological analyses and the test is used in many. However, now, the availability and quality of drinking water are questionable. Khan, abdul ghafoor pmrc central research centre, national institute of health, islamabad. The total viable counts for all water samples were quite high ranging from 6. In monitoring for attainment of the recreational use the appropriate indicator must be determined before sample collection. Drinking water bacteriological analysis request form samples over 30 hours old and not in approved container are unsuitable for testing sampling procedure. African journal of clinical and experimental microbiology. Get a printable copy pdf file of the complete article 150k, or click on a page image below to browse page by page. Domestic water is generally suppli ed to homes through private wells or public water com panies.
It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. It must be appreciated that all that bacteriological analysis can prove is that, at the time of examination, contamination or bacteria indicative of faecal pollution, could or could not be demonstrated in a given sample of water using specified culture methods. Chapter 6 through chapter 26 cover injurious or toxic substances of domestic, agricultural, and industrial sources. Water sample for analysis should be collected in sterile bottle made up of neutral glass of capacity 200500 ml. Bacteriological analyses were carried out on ananthanar channel water of kanyakumari district, tamil. The d1974 form and the final report from the certified lab that analyzed your samples is due to be reported to dhec on the 10th of each month. Enumeration methods membrane filter multiple tube fermentation 3. The quality of potable water and treatment of waterborne diseases are critical public health issues. Pdf bacteriological quality analysis of tap water of. Bacteriological analysis of water worksheet lab number read by. One of the techniques is membrane filtration method. Where we are today still using analytical microbiological methods developed over a century ago in the uk, good quality water is the expected norm more than 99. Bacterial examination of water the bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing, swimming and other domestic and industrial uses.
Water is one of the most precious commodities of life and society. Bacteriological quality of water samples in osogbo metropolis. Water sampling and analysis world health organization. Water samples, collected from different areas of delhi and ncr, have been analyzed for most probable number mpn test, as well as checked. A study of the bacteriological quality of bottled and tap. Bacteriological analysis of well water sources in the bambui student residential area. Many of these bacteria are capable of growing on a wide variety of single carbon sources. Microbiological analysis of drinking water quality of ananthanar channel of.
The complete analysis consists of series of test to identify the bacteria. Collection of water sample is important step in bacteriological examination of water. All coliforms will be detected at 37oc but only faecal coliforms at 44oc. Turn on the tap for maximum flow and the water may run for two minutes. The user is urged to check with the laboratory performing the analysis to. Laboratory staff for the collection of chemistry samples for drinking water analysis.
Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. Therefore adequate and safe water supply should be available to humans, plants, and animals in all parts of the world. A good bacterial indicator of fecal pollution should fulfill the following criteria. Other uses include recreational, surface or marine water, bottled water, groundwater, well water, treatment plant effluents. Bacteriological quality analysis of tap water of karachi, pakistan. Selecting an indicator for classified water bodies.
It is the only procedure that can be used if water samples are very turbid or if semisolids such as sediments or sludges are to be analysed. Abstract water is one of the most important constituents of life support system. Chapter 4 and chapter 5 discuss bacteriological and algal analysis. Bacteriological analysis of drinking water sources. Drinking water bacteriological analysis request form. Bacteriological analysis of drinking water sources article pdf available in african journal of microbiology research 518 september 2011 with 12,055 reads how we measure reads. The study was performed during the warm, rainy season, when prevalence of diarrhoea linked to bacterial contamination of water supply is usually at its highest level in tropical countries. Water of good drinking quality is of basic importance to human physiology as well as indispensable to mans con tinued existence 1. Microbiological analysis of drinking water quality of ananthanar channel of kanyakumari district, tamil nadu, india. The first, or presumptive test, is a screening test to sample water for the presence of coliform organisms. Hence, as described in chapter 1, the microbiological examination of drinkingwater emphasizes assessment of the hygienic quality of the supply. Food and drug administration laboratories for the detection in food and cosmetic.
Bacteriological analysis of water sampling precautions. The first of these is called the multiple fermentation tube or most probable number technique. The examination is intended to identify water sources which have been contaminated. Quick guide to drinking water sample collection second. System information to be completed by department of natural resourcessampler. In this method measured portions of a water sample are placed in testtubes containing a culture medium. Use only the laboratorysterilized bottle provided with sodium thiosulfate additive.
The research determines bacteriological quality of drinking water sources in serbo town, south west ethiopia. Bacteriological analysis of water using the pour plate method, total heterotrophic bacteria in the water samples were obtained dhawale and lamaster, 2003. Results of the bacteriological analysis of the water sample are presented in table 2. Dnr drinking water program public water supply rev. The examination of water supplies that have been disinfected with. Bacteriological analysis of water by multiple tube method. Data representation results obtained were represented using table and bar chart. Chain of custody drinking water bacteriological analysis. Bacteriological quality of different water samples are shown in figure 1.
This document is intended as a refresher for those already trained in sample collection. Sampling location identification code geographical location university female. Total coliforms and escherichia coli in water by membrane. Humans consume and pollute large quantities of water. Bacteriological analysis of water is performed to check the number of bacteria the water is contaminated with or simply it is a basic test for knowing about the water quality. Evaluation of bacteriological quality of surface, well. Analysis of results was done using microsoft excel package to explore and deduce the standard deviation and mean of the results as shown below. Remove any screens, filters or aerator devices from your faucet and allow the water to run for several minutes.
Pdf bacteriological analysis of drinking water sources. The quality of water is of vital concern for mankind since it is directly linked with human welfare. Bacteriological testing of water loughborough university. Borehole water, physical, chemical, bacteriological analysis, enterobacteriaceae. Clean and safe drinking water is the basic need of human beings. Please read all instructions before collecting water sample. The bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing, swimming and other domestic and industrial uses. This video tutorial demonstrates bacteriological analysis of water by multiple tube method. Water quality monitoring world health organization. Water contamination is one of the principal public health hazards associated with disasters, sinci contaminated water can set off an increase in gastroenteritis, diarrhea, and other waterborne diseases. This area for lab use only drinking water results sample bottles will often have plastic or paper seals on the cap, carefully remove this seal without touching the insides of the container.
Other approaches to sample collection may be acceptable or desirable under given conditions. Bio 3b laboratory bacteriological examination of water. Using a clean cloth outlet of the tap wipe to remove any dirt. The principal risk associated with water in small community supplies is that of infectious disease related to faecal contamination. Once initially for community wells pollution usually expected to bacteriological testing only. Abstract a total of 112 water samples in and around islamabad were assessed for bacteriological contamination. The term faecal coliform has been used in water microbiology to denote coliform. A physicochemical and bacteriological analysis of borehole water samples from the wa municipality of the upper west region, ghana godfred etsey sebiawu, samuel asiedu fosu and sixtus bieranye bayaa martin saana dispensing technology department, wa polytechnic, wa.
Pdf bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what. Physicochemical and bacteriological analyses of water used for. Physicochemical and bacteriological analyses of water. Sample collection procedure for bacteriological analysis of drinking water. The bacterial examination of water has been standardized into three tests. Fdas bacteriological analytical manual the bam is a collection of procedures preferred by analysts in u. Water contaminated with pathogenic species also has the normal inhabitants of the human intestine. Bacteriological water analysis online biology notes. Bacteriological water analysis is a method of analysing water to estimate the numbers of. Bacteriological analysis enclose form when sending sample to lab rev. Bacteriological analysis of water bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. Pathogen indicators coliform streptococcus enterococcus 3.
Bacteriological analysis of well water sources in the. Such contamination may take place at the source, at the. If sample collection process is not appropriate, it gives false result and raises question about validity and liability of the test. Introduction water is one of the most important necessities to all forms of life on this planet and beyond. Bacteriological analysis of drinking water for private.
Bacteriological analysis raw water pdf bromate analysis pdf bromide analysis pdf. Bacterial contamination of drinking water sources is the most common health risk. Suite 716 san diego, ca 92110 patricia mcvay, md, chief lab director signature date remarks approved revised signature date remarks. Disinfect the end of the faucet with an alcohol swab or dilute bleach solution 1 part household bleach to 10 parts water. The technique has been used for the analysis of drinkingwater for many years with satisfactory results. The aerobic gram negative rods of the genera pseudomonas, alcalignes and flavobacterium as well as others are common in water. Results bacteriological analysis of water samples from four sources. It is regularly tested for in coastal sea water samples, as well as runoff water. In our study for microbiological water quality we followed next methodology.
Bacteriological analysis of drinking water in zamfara. Comparative bacteriological analysis of stored borehole. Bio 3b laboratory, water quality page 1 of 3 bio 3b laboratory bacteriological examination of water objectives carry out a presumptive test for the presence of coliform bacteria in a water sample determine the most probable number mpn of bacteria in a positive presumptive sample. Microbiological analysis of drinking water quality of. Bacteriological analysis of drinking water for private citizen, single household only collecting your water sample 1.
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